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Screening self-peptides for recognition by mouse alloreactive CD8(+) T cells using direct ex vivo multimer staining
Here, we present a protocol to identify immunogenic self-peptide/allogeneic major histocompatibility complex (MHC) epitopes. We describe the generation of enriched alloreactive CD8+ T cells by priming mice with a skin graft expressing the allogeneic MHC class I molecule of interest, followed by boos...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792548/ https://www.ncbi.nlm.nih.gov/pubmed/36525346 http://dx.doi.org/10.1016/j.xpro.2022.101943 |