Screening self-peptides for recognition by mouse alloreactive CD8(+) T cells using direct ex vivo multimer staining

Here, we present a protocol to identify immunogenic self-peptide/allogeneic major histocompatibility complex (MHC) epitopes. We describe the generation of enriched alloreactive CD8+ T cells by priming mice with a skin graft expressing the allogeneic MHC class I molecule of interest, followed by boos...

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Detalles Bibliográficos
Autores principales: Son, Eric T., Paul-Heng, Moumita, Leong, Mario, Wang, Chuanmin, Hill, Alexandra E., Denkova, Martina, Purcell, Anthony W., Mifsud, Nicole A., Sharland, Alexandra F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792548/
https://www.ncbi.nlm.nih.gov/pubmed/36525346
http://dx.doi.org/10.1016/j.xpro.2022.101943

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