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Isolation of adoptively transferred CD8(+) T cells in mouse tumor tissues for lipid peroxidation detection

The lipid peroxidation level of tumor-infiltrating CD8(+) T cells is crucial for its activity and longevity. Here, we describe a protocol for effective and epitope-preserving dissociation of mouse tumors and subsequent leukocyte purification and lipid peroxidation staining of adoptively transferred...

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Detalles Bibliográficos
Autores principales: Xiao, Liuling, Yi, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792557/
https://www.ncbi.nlm.nih.gov/pubmed/36525345
http://dx.doi.org/10.1016/j.xpro.2022.101945