Adeno-associated viral delivery of engineered tRNA-enzyme pairs into nonsense mutation mouse models

In this protocol, we describe how to utilize the unnatural amino acid (UAA) incorporation system to read through endogenous premature termination codons in a Duchenne muscular dystrophy mouse model. We detail how to screen and optimize tRNA-enzyme pairs for efficient UAA incorporation, deliver the s...

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Detalles Bibliográficos
Autores principales: Zheng, Zhetao, Shi, Ningning, Xia, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792948/
https://www.ncbi.nlm.nih.gov/pubmed/36527714
http://dx.doi.org/10.1016/j.xpro.2022.101950

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792948/
https://www.ncbi.nlm.nih.gov/pubmed/36527714
http://dx.doi.org/10.1016/j.xpro.2022.101950

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