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Adeno-associated viral delivery of engineered tRNA-enzyme pairs into nonsense mutation mouse models
In this protocol, we describe how to utilize the unnatural amino acid (UAA) incorporation system to read through endogenous premature termination codons in a Duchenne muscular dystrophy mouse model. We detail how to screen and optimize tRNA-enzyme pairs for efficient UAA incorporation, deliver the s...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792948/ https://www.ncbi.nlm.nih.gov/pubmed/36527714 http://dx.doi.org/10.1016/j.xpro.2022.101950 |