Prediction and verification of glycosyltransferase activity by bioinformatics analysis and protein engineering

A significant number of proteins are annotated as functionally uncharacterized proteins. Within this protocol, we describe how to use protein family multiple sequence alignments and structural bioinformatics resources to design loss-of-function mutations of previously uncharacterized proteins within...

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Detalles Bibliográficos
Autores principales: Gerloff, Dietlind L., Ilina, Elena I., Cialini, Camille, Mata Salcedo, Uxue, Mittelbronn, Michel, Müller, Tanja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792956/
https://www.ncbi.nlm.nih.gov/pubmed/36528856
http://dx.doi.org/10.1016/j.xpro.2022.101905

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792956/
https://www.ncbi.nlm.nih.gov/pubmed/36528856
http://dx.doi.org/10.1016/j.xpro.2022.101905

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