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Prediction and verification of glycosyltransferase activity by bioinformatics analysis and protein engineering
A significant number of proteins are annotated as functionally uncharacterized proteins. Within this protocol, we describe how to use protein family multiple sequence alignments and structural bioinformatics resources to design loss-of-function mutations of previously uncharacterized proteins within...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9792956/ https://www.ncbi.nlm.nih.gov/pubmed/36528856 http://dx.doi.org/10.1016/j.xpro.2022.101905 |