A simple and efficient method for cytoplasmic production of human enterokinase light chain in E. coli

Human enterokinase light chain (hEKL) cDNA sequence was designed with the help of codon optimization towards Escherichia coli codon preference and ribosome binding site design and artificially synthesized with a thioredoxin fusion tag at the N-terminal and a five his-tag peptide at the C-terminal. T...

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Detalles Bibliográficos
Autores principales: Ebrahimifard, Mohammad, Forghanifard, Mohammad Mahdi, Yamchi, Ahad, Zarrinpour, Vajiheh, Sharbatkhari, Mahrokh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9794667/
https://www.ncbi.nlm.nih.gov/pubmed/36574134
http://dx.doi.org/10.1186/s13568-022-01504-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9794667/
https://www.ncbi.nlm.nih.gov/pubmed/36574134
http://dx.doi.org/10.1186/s13568-022-01504-9

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