Extracting and characterizing protein-free megabase-pair DNA for in vitro experiments

Chromosome structure and function is studied using various cell-based methods as well as with a range of in vitro single-molecule techniques on short DNA substrates. Here, we present a method to obtain megabase-pair-length deproteinated DNA for in vitro studies. We isolated chromosomes from bacteria...

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Detalles Bibliográficos
Autores principales: Holub, Martin, Birnie, Anthony, Japaridze, Aleksandre, van der Torre, Jaco, Ridder, Maxime den, de Ram, Carol, Pabst, Martin, Dekker, Cees
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795359/
https://www.ncbi.nlm.nih.gov/pubmed/36590691
http://dx.doi.org/10.1016/j.crmeth.2022.100366

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795359/
https://www.ncbi.nlm.nih.gov/pubmed/36590691
http://dx.doi.org/10.1016/j.crmeth.2022.100366

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