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Extracting and characterizing protein-free megabase-pair DNA for in vitro experiments
Chromosome structure and function is studied using various cell-based methods as well as with a range of in vitro single-molecule techniques on short DNA substrates. Here, we present a method to obtain megabase-pair-length deproteinated DNA for in vitro studies. We isolated chromosomes from bacteria...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795359/ https://www.ncbi.nlm.nih.gov/pubmed/36590691 http://dx.doi.org/10.1016/j.crmeth.2022.100366 |