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Protein fluorescent labeling in live yeast cells using scFv-based probes

The fusion of fluorescent proteins (FPs) to endogenous proteins is a widespread approach for microscopic examination of protein function, expression, and localization in the cell. However, proteins that are sensitive to FP fusion or expressed at low levels are difficult to monitor using this approac...

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Detalles Bibliográficos
Autores principales: Tsirkas, Ioannis, Zur, Tomer, Dovrat, Daniel, Cohen, Amit, Ravkaie, Lior, Aharoni, Amir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795370/
https://www.ncbi.nlm.nih.gov/pubmed/36590693
http://dx.doi.org/10.1016/j.crmeth.2022.100357