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Protein fluorescent labeling in live yeast cells using scFv-based probes
The fusion of fluorescent proteins (FPs) to endogenous proteins is a widespread approach for microscopic examination of protein function, expression, and localization in the cell. However, proteins that are sensitive to FP fusion or expressed at low levels are difficult to monitor using this approac...
Autores principales: | Tsirkas, Ioannis, Zur, Tomer, Dovrat, Daniel, Cohen, Amit, Ravkaie, Lior, Aharoni, Amir |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795370/ https://www.ncbi.nlm.nih.gov/pubmed/36590693 http://dx.doi.org/10.1016/j.crmeth.2022.100357 |
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