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Live-cell imaging and analysis of actin-mediated mitochondrial fission

Current approaches, such as fixed-cell imaging or single-snapshot imaging, are insufficient to capture cytoskeleton-mediated mitochondrial fission. Here, we present a protocol to capture actin-mediated mitochondrial fission using high-resolution time-lapse imaging. We describe steps starting from ce...

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Detalles Bibliográficos
Autores principales: Shimura, Daisuke, Shaw, Robin M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9795527/
https://www.ncbi.nlm.nih.gov/pubmed/36542522
http://dx.doi.org/10.1016/j.xpro.2022.101958