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Focus variation due to near infrared laser in a confocal microscope

Focus precision and stability is crucial in confocal microscopy not only for image sharpness but also to avoid radiometric fluctuations that can wrongly be interpreted as variations of the fluorescence intensity in the sample. Here we report a focus variation provoked by a continuous wave laser of 8...

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Detalles Bibliográficos
Autores principales: Matrecitos‐Avila, Melissa, Avila, Remy, Pimentel‐Domínguez, Reinher, Cuevas, Salvador, Tamariz, Elisa, Loza‐Alvarez, Pablo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796951/
https://www.ncbi.nlm.nih.gov/pubmed/35819025
http://dx.doi.org/10.1002/jemt.24198