The effect of a long-term treatment with cannabidiol-rich hemp extract oil on the adenosinergic system of the zucker diabetic fatty (ZDF) rat atrium

Cannabidiol (CBD), the most extensively studied non-intoxicating phytocannabinoid, has been attracting a lot of interest worldwide owing to its numerous beneficial effects. The aim of this study was to explore the effect that CBD exerts on the adenosinergic system of paced left atria isolated from o...

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Autores principales: Viczjan, Gabor, Szilagyi, Anna, Takacs, Barbara, Ovari, Ignac, Szekeres, Reka, Tarjanyi, Vera, Erdei, Tamas, Teleki, Vanda, Zsuga, Judit, Szilvassy, Zoltan, Juhasz, Bela, Varga, Balazs, Gesztelyi, Rudolf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797669/
https://www.ncbi.nlm.nih.gov/pubmed/36588715
http://dx.doi.org/10.3389/fphar.2022.1043275
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author Viczjan, Gabor
Szilagyi, Anna
Takacs, Barbara
Ovari, Ignac
Szekeres, Reka
Tarjanyi, Vera
Erdei, Tamas
Teleki, Vanda
Zsuga, Judit
Szilvassy, Zoltan
Juhasz, Bela
Varga, Balazs
Gesztelyi, Rudolf
author_facet Viczjan, Gabor
Szilagyi, Anna
Takacs, Barbara
Ovari, Ignac
Szekeres, Reka
Tarjanyi, Vera
Erdei, Tamas
Teleki, Vanda
Zsuga, Judit
Szilvassy, Zoltan
Juhasz, Bela
Varga, Balazs
Gesztelyi, Rudolf
author_sort Viczjan, Gabor
collection PubMed
description Cannabidiol (CBD), the most extensively studied non-intoxicating phytocannabinoid, has been attracting a lot of interest worldwide owing to its numerous beneficial effects. The aim of this study was to explore the effect that CBD exerts on the adenosinergic system of paced left atria isolated from obese type Zucker Diabetic Fatty (ZDF) rats, maintained on diabetogenic rat chow, received 60 mg/kg/day CBD or vehicle via gavage for 4 weeks. We found that N6-cyclopentyladenosine (CPA), a relatively stable and poorly transported A1 adenosine receptor agonist, elicited a significantly weaker response in the CBD-treated group than in the vehicle-treated one. In contrast, adenosine, a quickly metabolized and transported adenosine receptor agonist, evoked a significantly stronger response in the CBD-treated group than in the vehicle-treated counterpart (excepting its highest concentrations). These results can be explained only with the adenosine transport inhibitory property of CBD (and not with its adenosine receptor agonist activity). If all the effects of CBD are attributed to the interstitial adenosine accumulation caused by CBD in the myocardium, then a significantly increased adenosinergic activation can be assumed during the long-term oral CBD treatment, suggesting a considerably enhanced adenosinergic protection in the heart. Considering that our results may have been influenced by A1 adenosine receptor downregulation due to the chronic interstitial adenosine accumulation, an adenosinergic activation smaller than it seemed cannot be excluded, but it was above the CBD-naïve level in every case. Additionally, this is the first study offering functional evidence about the adenosine transport inhibitory action of CBD in the myocardium.
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spelling pubmed-97976692022-12-30 The effect of a long-term treatment with cannabidiol-rich hemp extract oil on the adenosinergic system of the zucker diabetic fatty (ZDF) rat atrium Viczjan, Gabor Szilagyi, Anna Takacs, Barbara Ovari, Ignac Szekeres, Reka Tarjanyi, Vera Erdei, Tamas Teleki, Vanda Zsuga, Judit Szilvassy, Zoltan Juhasz, Bela Varga, Balazs Gesztelyi, Rudolf Front Pharmacol Pharmacology Cannabidiol (CBD), the most extensively studied non-intoxicating phytocannabinoid, has been attracting a lot of interest worldwide owing to its numerous beneficial effects. The aim of this study was to explore the effect that CBD exerts on the adenosinergic system of paced left atria isolated from obese type Zucker Diabetic Fatty (ZDF) rats, maintained on diabetogenic rat chow, received 60 mg/kg/day CBD or vehicle via gavage for 4 weeks. We found that N6-cyclopentyladenosine (CPA), a relatively stable and poorly transported A1 adenosine receptor agonist, elicited a significantly weaker response in the CBD-treated group than in the vehicle-treated one. In contrast, adenosine, a quickly metabolized and transported adenosine receptor agonist, evoked a significantly stronger response in the CBD-treated group than in the vehicle-treated counterpart (excepting its highest concentrations). These results can be explained only with the adenosine transport inhibitory property of CBD (and not with its adenosine receptor agonist activity). If all the effects of CBD are attributed to the interstitial adenosine accumulation caused by CBD in the myocardium, then a significantly increased adenosinergic activation can be assumed during the long-term oral CBD treatment, suggesting a considerably enhanced adenosinergic protection in the heart. Considering that our results may have been influenced by A1 adenosine receptor downregulation due to the chronic interstitial adenosine accumulation, an adenosinergic activation smaller than it seemed cannot be excluded, but it was above the CBD-naïve level in every case. Additionally, this is the first study offering functional evidence about the adenosine transport inhibitory action of CBD in the myocardium. Frontiers Media S.A. 2022-12-15 /pmc/articles/PMC9797669/ /pubmed/36588715 http://dx.doi.org/10.3389/fphar.2022.1043275 Text en Copyright © 2022 Viczjan, Szilagyi, Takacs, Ovari, Szekeres, Tarjanyi, Erdei, Teleki, Zsuga, Szilvassy, Juhasz, Varga and Gesztelyi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Viczjan, Gabor
Szilagyi, Anna
Takacs, Barbara
Ovari, Ignac
Szekeres, Reka
Tarjanyi, Vera
Erdei, Tamas
Teleki, Vanda
Zsuga, Judit
Szilvassy, Zoltan
Juhasz, Bela
Varga, Balazs
Gesztelyi, Rudolf
The effect of a long-term treatment with cannabidiol-rich hemp extract oil on the adenosinergic system of the zucker diabetic fatty (ZDF) rat atrium
title The effect of a long-term treatment with cannabidiol-rich hemp extract oil on the adenosinergic system of the zucker diabetic fatty (ZDF) rat atrium
title_full The effect of a long-term treatment with cannabidiol-rich hemp extract oil on the adenosinergic system of the zucker diabetic fatty (ZDF) rat atrium
title_fullStr The effect of a long-term treatment with cannabidiol-rich hemp extract oil on the adenosinergic system of the zucker diabetic fatty (ZDF) rat atrium
title_full_unstemmed The effect of a long-term treatment with cannabidiol-rich hemp extract oil on the adenosinergic system of the zucker diabetic fatty (ZDF) rat atrium
title_short The effect of a long-term treatment with cannabidiol-rich hemp extract oil on the adenosinergic system of the zucker diabetic fatty (ZDF) rat atrium
title_sort effect of a long-term treatment with cannabidiol-rich hemp extract oil on the adenosinergic system of the zucker diabetic fatty (zdf) rat atrium
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797669/
https://www.ncbi.nlm.nih.gov/pubmed/36588715
http://dx.doi.org/10.3389/fphar.2022.1043275
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