Next-Generation Digital Polymerase Chain Reaction: High-Dynamic-Range Single-Molecule DNA Counting via Ultrapartitioning

[Image: see text] Digital PCR (dPCR) was first conceived for single-molecule quantitation. However, current dPCR systems often require DNA templates to share partitions due to limited partitioning capacities. Here, we introduce UltraPCR, a next-generation dPCR system where DNA counting is performed...

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Detalles Bibliográficos
Autores principales: Shum, Eleen Y., Lai, Janice H., Li, Sixing, Lee, Haeun G., Soliman, Jesse, Raol, Vedant K., Lee, Cavina K., Fodor, Stephen P.A., Fan, H. Christina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798378/
https://www.ncbi.nlm.nih.gov/pubmed/36508568
http://dx.doi.org/10.1021/acs.analchem.2c03649

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798378/
https://www.ncbi.nlm.nih.gov/pubmed/36508568
http://dx.doi.org/10.1021/acs.analchem.2c03649

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