Cargando…

Progranulin from different gliocytes in the nucleus accumbens exerts distinct roles in FTD- and neuroinflammation-induced depression-like behaviors

BACKGROUND: Neuroinflammation in the nucleus accumbens (NAc) is well known to influence the progression of depression. However, the molecular mechanisms triggering NAc neuroinflammation in depression have not been fully elucidated. Progranulin (PGRN) is a multifunctional growth factor that is linked...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Jing, Lai, Simin, Zhou, Ting, Xia, Zhihao, Li, Weina, Sha, Wenqi, Liu, Jingjie, Chen, Yanjiong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798954/
https://www.ncbi.nlm.nih.gov/pubmed/36581897
http://dx.doi.org/10.1186/s12974-022-02684-8
_version_ 1784861013424734208
author Wang, Jing
Lai, Simin
Zhou, Ting
Xia, Zhihao
Li, Weina
Sha, Wenqi
Liu, Jingjie
Chen, Yanjiong
author_facet Wang, Jing
Lai, Simin
Zhou, Ting
Xia, Zhihao
Li, Weina
Sha, Wenqi
Liu, Jingjie
Chen, Yanjiong
author_sort Wang, Jing
collection PubMed
description BACKGROUND: Neuroinflammation in the nucleus accumbens (NAc) is well known to influence the progression of depression. However, the molecular mechanisms triggering NAc neuroinflammation in depression have not been fully elucidated. Progranulin (PGRN) is a multifunctional growth factor that is linked to the innate immune response and inflammation, and PGRN plays a key role in neurodegenerative diseases such as frontotemporal dementia (FTD). Here, the purpose of this study was to validate whether PGRN was involved in the NAc neuroinflammation-promoted depressive-like phenotype. METHODS: A NAc neuroinflammation-relevant depression-like model was established using wild-type (WT) and PGRN-knockout (KO) mice after NAc injection with lipopolysaccharide (LPS), and various behavioral tests related to cognition, social recognition, depression and anxiety were performed with WT and PGRNKO mice with or without NAc immune challenge. RT‒PCR, ELISA, western blotting and immunofluorescence staining were used to determine the expression and function of PGRN in the neuroinflammatory reaction in the NAc after LPS challenge. The morphology of neurons in the NAc from WT and PGRNKO mice under conditions of NAc neuroinflammation was analyzed using Golgi–Cox staining, followed by Sholl analyses. The potential signaling pathways involved in NAc neuroinflammation in PGRNKO mice were investigated by western blotting. RESULTS: Under normal conditions, PGRN deficiency induced FTD-like behaviors in mice and astrocyte activation in the NAc, promoted the release of the inflammatory cytokines interleukin (IL)-6 and IL-10 and increased dendritic complexity and synaptic protein BDNF levels in the NAc. However, NAc neuroinflammation enhanced PGRN expression, which was located in astrocytes and microglia within the NAc, and PGRN deficiency in mice alleviated NAc neuroinflammation-elicited depression-like behaviors, seemingly inhibiting astrocyte- and microglia-related inflammatory reactions and neuroplasticity complexity in the NAc via the p38 and nuclear factor of kappa (NF-κB) signaling pathways present in the NAc after neuroinflammation. CONCLUSIONS: Our results suggest that PGRN exerts distinct function on different behaviors, showing protective roles in the FTD-like behavior and detrimental effects on the neuroinflammation-related depression-like behavior, resulting from mediating astrocyte and microglial functions from the NAc in different status. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12974-022-02684-8.
format Online
Article
Text
id pubmed-9798954
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-97989542022-12-30 Progranulin from different gliocytes in the nucleus accumbens exerts distinct roles in FTD- and neuroinflammation-induced depression-like behaviors Wang, Jing Lai, Simin Zhou, Ting Xia, Zhihao Li, Weina Sha, Wenqi Liu, Jingjie Chen, Yanjiong J Neuroinflammation Research BACKGROUND: Neuroinflammation in the nucleus accumbens (NAc) is well known to influence the progression of depression. However, the molecular mechanisms triggering NAc neuroinflammation in depression have not been fully elucidated. Progranulin (PGRN) is a multifunctional growth factor that is linked to the innate immune response and inflammation, and PGRN plays a key role in neurodegenerative diseases such as frontotemporal dementia (FTD). Here, the purpose of this study was to validate whether PGRN was involved in the NAc neuroinflammation-promoted depressive-like phenotype. METHODS: A NAc neuroinflammation-relevant depression-like model was established using wild-type (WT) and PGRN-knockout (KO) mice after NAc injection with lipopolysaccharide (LPS), and various behavioral tests related to cognition, social recognition, depression and anxiety were performed with WT and PGRNKO mice with or without NAc immune challenge. RT‒PCR, ELISA, western blotting and immunofluorescence staining were used to determine the expression and function of PGRN in the neuroinflammatory reaction in the NAc after LPS challenge. The morphology of neurons in the NAc from WT and PGRNKO mice under conditions of NAc neuroinflammation was analyzed using Golgi–Cox staining, followed by Sholl analyses. The potential signaling pathways involved in NAc neuroinflammation in PGRNKO mice were investigated by western blotting. RESULTS: Under normal conditions, PGRN deficiency induced FTD-like behaviors in mice and astrocyte activation in the NAc, promoted the release of the inflammatory cytokines interleukin (IL)-6 and IL-10 and increased dendritic complexity and synaptic protein BDNF levels in the NAc. However, NAc neuroinflammation enhanced PGRN expression, which was located in astrocytes and microglia within the NAc, and PGRN deficiency in mice alleviated NAc neuroinflammation-elicited depression-like behaviors, seemingly inhibiting astrocyte- and microglia-related inflammatory reactions and neuroplasticity complexity in the NAc via the p38 and nuclear factor of kappa (NF-κB) signaling pathways present in the NAc after neuroinflammation. CONCLUSIONS: Our results suggest that PGRN exerts distinct function on different behaviors, showing protective roles in the FTD-like behavior and detrimental effects on the neuroinflammation-related depression-like behavior, resulting from mediating astrocyte and microglial functions from the NAc in different status. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12974-022-02684-8. BioMed Central 2022-12-29 /pmc/articles/PMC9798954/ /pubmed/36581897 http://dx.doi.org/10.1186/s12974-022-02684-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Jing
Lai, Simin
Zhou, Ting
Xia, Zhihao
Li, Weina
Sha, Wenqi
Liu, Jingjie
Chen, Yanjiong
Progranulin from different gliocytes in the nucleus accumbens exerts distinct roles in FTD- and neuroinflammation-induced depression-like behaviors
title Progranulin from different gliocytes in the nucleus accumbens exerts distinct roles in FTD- and neuroinflammation-induced depression-like behaviors
title_full Progranulin from different gliocytes in the nucleus accumbens exerts distinct roles in FTD- and neuroinflammation-induced depression-like behaviors
title_fullStr Progranulin from different gliocytes in the nucleus accumbens exerts distinct roles in FTD- and neuroinflammation-induced depression-like behaviors
title_full_unstemmed Progranulin from different gliocytes in the nucleus accumbens exerts distinct roles in FTD- and neuroinflammation-induced depression-like behaviors
title_short Progranulin from different gliocytes in the nucleus accumbens exerts distinct roles in FTD- and neuroinflammation-induced depression-like behaviors
title_sort progranulin from different gliocytes in the nucleus accumbens exerts distinct roles in ftd- and neuroinflammation-induced depression-like behaviors
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798954/
https://www.ncbi.nlm.nih.gov/pubmed/36581897
http://dx.doi.org/10.1186/s12974-022-02684-8
work_keys_str_mv AT wangjing progranulinfromdifferentgliocytesinthenucleusaccumbensexertsdistinctrolesinftdandneuroinflammationinduceddepressionlikebehaviors
AT laisimin progranulinfromdifferentgliocytesinthenucleusaccumbensexertsdistinctrolesinftdandneuroinflammationinduceddepressionlikebehaviors
AT zhouting progranulinfromdifferentgliocytesinthenucleusaccumbensexertsdistinctrolesinftdandneuroinflammationinduceddepressionlikebehaviors
AT xiazhihao progranulinfromdifferentgliocytesinthenucleusaccumbensexertsdistinctrolesinftdandneuroinflammationinduceddepressionlikebehaviors
AT liweina progranulinfromdifferentgliocytesinthenucleusaccumbensexertsdistinctrolesinftdandneuroinflammationinduceddepressionlikebehaviors
AT shawenqi progranulinfromdifferentgliocytesinthenucleusaccumbensexertsdistinctrolesinftdandneuroinflammationinduceddepressionlikebehaviors
AT liujingjie progranulinfromdifferentgliocytesinthenucleusaccumbensexertsdistinctrolesinftdandneuroinflammationinduceddepressionlikebehaviors
AT chenyanjiong progranulinfromdifferentgliocytesinthenucleusaccumbensexertsdistinctrolesinftdandneuroinflammationinduceddepressionlikebehaviors