Neutron crystallography and quantum chemical analysis of bilin reductase PcyA mutants reveal substrate and catalytic residue protonation states

PcyA, a ferredoxin-dependent bilin pigment reductase, catalyzes the site-specific reduction of the two vinyl groups of biliverdin (BV), producing phycocyanobilin. Previous neutron crystallography detected both the neutral BV and its protonated form (BVH(+)) in the wildtype (WT) PcyA–BV complex, and...

Descripción completa

Detalles Bibliográficos
Autores principales: Joutsuka, Tatsuya, Nanasawa, Ryota, Igarashi, Keisuke, Horie, Kazuki, Sugishima, Masakazu, Hagiwara, Yoshinori, Wada, Kei, Fukuyama, Keiichi, Yano, Naomine, Mori, Seiji, Ostermann, Andreas, Kusaka, Katsuhiro, Unno, Masaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800206/
https://www.ncbi.nlm.nih.gov/pubmed/36463961
http://dx.doi.org/10.1016/j.jbc.2022.102763

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800206/
https://www.ncbi.nlm.nih.gov/pubmed/36463961
http://dx.doi.org/10.1016/j.jbc.2022.102763

Ejemplares similares