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Neutron crystallography and quantum chemical analysis of bilin reductase PcyA mutants reveal substrate and catalytic residue protonation states

PcyA, a ferredoxin-dependent bilin pigment reductase, catalyzes the site-specific reduction of the two vinyl groups of biliverdin (BV), producing phycocyanobilin. Previous neutron crystallography detected both the neutral BV and its protonated form (BVH(+)) in the wildtype (WT) PcyA–BV complex, and...

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Detalles Bibliográficos
Autores principales:	Joutsuka, Tatsuya, Nanasawa, Ryota, Igarashi, Keisuke, Horie, Kazuki, Sugishima, Masakazu, Hagiwara, Yoshinori, Wada, Kei, Fukuyama, Keiichi, Yano, Naomine, Mori, Seiji, Ostermann, Andreas, Kusaka, Katsuhiro, Unno, Masaki
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800206/ https://www.ncbi.nlm.nih.gov/pubmed/36463961 http://dx.doi.org/10.1016/j.jbc.2022.102763

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