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Effects of DNA extraction methods on the real time PCR quantification of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in chicken feces and ceca contents

Polymerase chain reaction (PCR) method was coupled with a DNA extraction to enumerate Campylobacter spp. from poultry gastrointestinal tract samples. Three experiments were conducted that included: 1) Development of a DNA standard curve related to bacterial DNA primers; 2) Design of a cell/genomic D...

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Detalles Bibliográficos
Autores principales: Dawson, Paul, Buyukyavuz, Ahmet, Ionita, Claudia, Northcutt, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800320/
https://www.ncbi.nlm.nih.gov/pubmed/36565641
http://dx.doi.org/10.1016/j.psj.2022.102369