Kinetics of DNA strand transfer between polymerase and proofreading exonuclease active sites regulates error correction during high-fidelity replication

We show that T7 DNA polymerase (pol) and exonuclease (exo) domains contribute to selective error correction during DNA replication by regulating bidirectional strand transfer between the two active sites. To explore the kinetic basis for selective removal of mismatches, we used a fluorescent cytosin...

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Detalles Bibliográficos
Autores principales: Dangerfield, Tyler L., Johnson, Kenneth A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800556/
https://www.ncbi.nlm.nih.gov/pubmed/36436560
http://dx.doi.org/10.1016/j.jbc.2022.102744

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800556/
https://www.ncbi.nlm.nih.gov/pubmed/36436560
http://dx.doi.org/10.1016/j.jbc.2022.102744

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