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Acylation driven by intracellular metabolites in host cells inhibits Cas9 activity used for genome editing
CRISPR-Cas, the immune system of bacteria and archaea, has been widely harnessed for genome editing, including gene knockouts and knockins, single-base editing, gene activation, and silencing. However, the molecular mechanisms underlying fluctuations in the genome editing efficiency of crispr in var...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9802096/ https://www.ncbi.nlm.nih.gov/pubmed/36712324 http://dx.doi.org/10.1093/pnasnexus/pgac277 |