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Executing cell-specific cross-linking immunoprecipitation and sequencing (seCLIP) in C. elegans

The single-end enhanced cross-linking immunoprecipitation (seCLIP) method is well suited for efficient and unbiased transcriptome-wide interrogation of RNA-binding protein (RBP) interaction sites. Here, we provide a protocol for executing cell-specific seCLIP for any desired RBP in Caenorhabditis el...

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Detalles Bibliográficos
Autores principales: Blazie, Stephen M., Jin, Yishi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803825/
https://www.ncbi.nlm.nih.gov/pubmed/36566382
http://dx.doi.org/10.1016/j.xpro.2022.101959