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Rational Control of Off‐State Heterogeneity in a Photoswitchable Fluorescent Protein Provides Switching Contrast Enhancement

Reversibly photoswitchable fluorescent proteins are essential markers for advanced biological imaging, and optimization of their photophysical properties underlies improved performance and novel applications. Here we establish a link between photoswitching contrast, one of the key parameters that di...

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Detalles Bibliográficos
Autores principales: Adam, Virgile, Hadjidemetriou, Kyprianos, Jensen, Nickels, Shoeman, Robert L., Woodhouse, Joyce, Aquila, Andrew, Banneville, Anne‐Sophie, Barends, Thomas R. M., Bezchastnov, Victor, Boutet, Sébastien, Byrdin, Martin, Cammarata, Marco, Carbajo, Sergio, Eleni Christou, Nina, Coquelle, Nicolas, De la Mora, Eugenio, El Khatib, Mariam, Moreno Chicano, Tadeo, Bruce Doak, R., Fieschi, Franck, Foucar, Lutz, Glushonkov, Oleksandr, Gorel, Alexander, Grünbein, Marie Luise, Hilpert, Mario, Hunter, Mark, Kloos, Marco, Koglin, Jason E., Lane, Thomas J., Liang, Mengning, Mantovanelli, Angela, Nass, Karol, Nass Kovacs, Gabriela, Owada, Shigeki, Roome, Christopher M., Schirò, Giorgio, Seaberg, Matthew, Stricker, Miriam, Thépaut, Michel, Tono, Kensuke, Ueda, Kiyoshi, Uriarte, Lucas M., You, Daehyun, Zala, Ninon, Domratcheva, Tatiana, Jakobs, Stefan, Sliwa, Michel, Schlichting, Ilme, Colletier, Jacques‐Philippe, Bourgeois, Dominique, Weik, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804819/
https://www.ncbi.nlm.nih.gov/pubmed/35959919
http://dx.doi.org/10.1002/cphc.202200192