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Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells

Several studies have indicated a role for cathepsin L (CTSL) proteolytic activity in the nucleus under distinct cellular conditions, including during differentiation, senescence, and quiescence. Here we show that addition of CTSL inhibitors to a cell lysis buffer prevents the cleavage of several nuc...

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Detalles Bibliográficos
Autores principales: Gaikwad, Prashant, Kemp, Michael G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807461/
https://www.ncbi.nlm.nih.gov/pubmed/36606083
http://dx.doi.org/10.17912/micropub.biology.000716
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author Gaikwad, Prashant
Kemp, Michael G.
author_facet Gaikwad, Prashant
Kemp, Michael G.
author_sort Gaikwad, Prashant
collection PubMed
description Several studies have indicated a role for cathepsin L (CTSL) proteolytic activity in the nucleus under distinct cellular conditions, including during differentiation, senescence, and quiescence. Here we show that addition of CTSL inhibitors to a cell lysis buffer prevents the cleavage of several nuclear proteins during the lysis of quiescent human cells, including proteins previously thought to have functional relevance in other cell and tissue contexts. These findings suggest that care should be taken to use CTSL inhibitors when lysing cells and tissues containing high levels of CTSL protein to differentiate proteolysis that occurs in vivo versus artifactually in vitro.
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spelling pubmed-98074612023-01-04 Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells Gaikwad, Prashant Kemp, Michael G. MicroPubl Biol New Finding Several studies have indicated a role for cathepsin L (CTSL) proteolytic activity in the nucleus under distinct cellular conditions, including during differentiation, senescence, and quiescence. Here we show that addition of CTSL inhibitors to a cell lysis buffer prevents the cleavage of several nuclear proteins during the lysis of quiescent human cells, including proteins previously thought to have functional relevance in other cell and tissue contexts. These findings suggest that care should be taken to use CTSL inhibitors when lysing cells and tissues containing high levels of CTSL protein to differentiate proteolysis that occurs in vivo versus artifactually in vitro. Caltech Library 2022-12-14 /pmc/articles/PMC9807461/ /pubmed/36606083 http://dx.doi.org/10.17912/micropub.biology.000716 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Finding
Gaikwad, Prashant
Kemp, Michael G.
Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells
title Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells
title_full Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells
title_fullStr Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells
title_full_unstemmed Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells
title_short Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells
title_sort cathepsin l inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells
topic New Finding
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807461/
https://www.ncbi.nlm.nih.gov/pubmed/36606083
http://dx.doi.org/10.17912/micropub.biology.000716
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