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Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells
Several studies have indicated a role for cathepsin L (CTSL) proteolytic activity in the nucleus under distinct cellular conditions, including during differentiation, senescence, and quiescence. Here we show that addition of CTSL inhibitors to a cell lysis buffer prevents the cleavage of several nuc...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807461/ https://www.ncbi.nlm.nih.gov/pubmed/36606083 http://dx.doi.org/10.17912/micropub.biology.000716 |
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author | Gaikwad, Prashant Kemp, Michael G. |
author_facet | Gaikwad, Prashant Kemp, Michael G. |
author_sort | Gaikwad, Prashant |
collection | PubMed |
description | Several studies have indicated a role for cathepsin L (CTSL) proteolytic activity in the nucleus under distinct cellular conditions, including during differentiation, senescence, and quiescence. Here we show that addition of CTSL inhibitors to a cell lysis buffer prevents the cleavage of several nuclear proteins during the lysis of quiescent human cells, including proteins previously thought to have functional relevance in other cell and tissue contexts. These findings suggest that care should be taken to use CTSL inhibitors when lysing cells and tissues containing high levels of CTSL protein to differentiate proteolysis that occurs in vivo versus artifactually in vitro. |
format | Online Article Text |
id | pubmed-9807461 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-98074612023-01-04 Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells Gaikwad, Prashant Kemp, Michael G. MicroPubl Biol New Finding Several studies have indicated a role for cathepsin L (CTSL) proteolytic activity in the nucleus under distinct cellular conditions, including during differentiation, senescence, and quiescence. Here we show that addition of CTSL inhibitors to a cell lysis buffer prevents the cleavage of several nuclear proteins during the lysis of quiescent human cells, including proteins previously thought to have functional relevance in other cell and tissue contexts. These findings suggest that care should be taken to use CTSL inhibitors when lysing cells and tissues containing high levels of CTSL protein to differentiate proteolysis that occurs in vivo versus artifactually in vitro. Caltech Library 2022-12-14 /pmc/articles/PMC9807461/ /pubmed/36606083 http://dx.doi.org/10.17912/micropub.biology.000716 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Gaikwad, Prashant Kemp, Michael G. Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells |
title | Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells |
title_full | Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells |
title_fullStr | Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells |
title_full_unstemmed | Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells |
title_short | Cathepsin L inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells |
title_sort | cathepsin l inhibition prevents the cleavage of multiple nuclear proteins upon lysis of quiescent human cells |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807461/ https://www.ncbi.nlm.nih.gov/pubmed/36606083 http://dx.doi.org/10.17912/micropub.biology.000716 |
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