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i-tRAP (individual tRNA acylation PCR): a convenient method for selective quantification of tRNA charging

Each transfer RNA (tRNA) is aminoacylated (charged) with a genetic codon-specific amino acid at its 3′ end. Charged tRNAs are primarily used for translation, whereas fluctuations in charged tRNA fractions are known to reflect cellular response to stress. Here we report the development of individual...

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Detalles Bibliográficos
Autores principales: Tsukamoto, Yusuke, Nakamura, Yumi, Hirata, Makoto, Sakate, Ryuichi, Kimura, Tomonori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9808569/
https://www.ncbi.nlm.nih.gov/pubmed/36283829
http://dx.doi.org/10.1261/rna.079323.122