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i-tRAP (individual tRNA acylation PCR): a convenient method for selective quantification of tRNA charging
Each transfer RNA (tRNA) is aminoacylated (charged) with a genetic codon-specific amino acid at its 3′ end. Charged tRNAs are primarily used for translation, whereas fluctuations in charged tRNA fractions are known to reflect cellular response to stress. Here we report the development of individual...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9808569/ https://www.ncbi.nlm.nih.gov/pubmed/36283829 http://dx.doi.org/10.1261/rna.079323.122 |