Negative autoregulation mitigates collateral RNase activity of repeat-targeting CRISPR-Cas13d in mammalian cells

CRISPR-Cas13 RNA endonucleases show promise for programmable RNA knockdown. However, sequence-specific binding of Cas13 unleashes non-specific bystander RNA cleavage, or collateral activity, raising concerns for experiments and therapeutic applications. Although robust in cell-free and bacterial env...

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Detalles Bibliográficos
Autores principales: Kelley, Chase P., Haerle, Maja C., Wang, Eric T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9809062/
https://www.ncbi.nlm.nih.gov/pubmed/35977479
http://dx.doi.org/10.1016/j.celrep.2022.111226

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9809062/
https://www.ncbi.nlm.nih.gov/pubmed/35977479
http://dx.doi.org/10.1016/j.celrep.2022.111226

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