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Microbial DNA extraction of high-host content and low biomass samples: Optimized protocol for nasopharynx metagenomic studies

INTRODUCTION: Low microbial biomass and high human DNA content in nasopharyngeal aspirate samples hinder comprehensive characterization of microbiota and resistome. We obtained samples from premature infants, a group with increased risk of developing respiratory disorders and infections, and consequ...

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Detalles Bibliográficos
Autores principales: Rajar, Polona, Dhariwal, Achal, Salvadori, Gabriela, Junges, Roger, Åmdal, Heidi Aarø, Berild, Dag, Fugelseth, Drude, Saugstad, Ola Didrik, Lausten-Thomsen, Ulrik, Greisen, Gorm, Haaland, Kirsti, Petersen, Fernanda Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9811202/
https://www.ncbi.nlm.nih.gov/pubmed/36620054
http://dx.doi.org/10.3389/fmicb.2022.1038120