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In Vitro Experimental Assessment of Ethanolic Extract of Moringa oleifera Leaves as an α-Amylase and α-Lipase Inhibitor

METHODS: Phytochemical screening, antioxidant activity, α-amylase, and α-lipase inhibitory assessment were carried out on Moringa oleifera extract. RESULTS: The result of the phytochemical screening revealed the presence of total phenolic, flavonoid, tannin, and alkaloid contents of values 0.070 ± 0...

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Autores principales: Ogundipe, Adebanke, Adetuyi, Babatunde, Iheagwam, Franklyn, Adefoyeke, Keleko, Olugbuyiro, Joseph, Ogunlana, Oluseyi, Ogunlana, Olubanke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9815922/
https://www.ncbi.nlm.nih.gov/pubmed/36620201
http://dx.doi.org/10.1155/2022/4613109
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author Ogundipe, Adebanke
Adetuyi, Babatunde
Iheagwam, Franklyn
Adefoyeke, Keleko
Olugbuyiro, Joseph
Ogunlana, Oluseyi
Ogunlana, Olubanke
author_facet Ogundipe, Adebanke
Adetuyi, Babatunde
Iheagwam, Franklyn
Adefoyeke, Keleko
Olugbuyiro, Joseph
Ogunlana, Oluseyi
Ogunlana, Olubanke
author_sort Ogundipe, Adebanke
collection PubMed
description METHODS: Phytochemical screening, antioxidant activity, α-amylase, and α-lipase inhibitory assessment were carried out on Moringa oleifera extract. RESULTS: The result of the phytochemical screening revealed the presence of total phenolic, flavonoid, tannin, and alkaloid contents of values 0.070 ± 0.005 mg gallic acid equivalent/g, 0.180 ± 0.020 mg rutin equivalent/g, 0.042 ± 0.001 mg tannic equivalent/g, and 12.17 ± 0.001%, respectively, while the total protein analysis was 0.475 ± 0.001 mg bovine serum albumin equivalent/g. Ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) values were 0.534 ± 0.001 mg gallic acid equivalent/g and 0.022 ± 0.00008 mg rutin equivalent/g, respectively. Diphenyl-2-picrylhydrazyl (DPPH), ABTS (2,2′-azino-bis (ethylbenzothiazoline-6-sulfonic acid)), and nitric oxide (NO) assays showed the extract to have a strong free radical scavenging activity. The 50% inhibitory concentration (IC(50)) values of the lipase and amylase activities of the extract are 1.0877 mg/mL and 0.1802 mg/mL, respectively. CONCLUSION: However, α-lipase and α-amylase inhibiting activity of M. oleifera could be related to the phytochemicals in the extract. This research validates the ethnobotanical use of M. oleifera leaves as an effective plant-based therapeutic agent for diabetes and obesity.
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spelling pubmed-98159222023-01-06 In Vitro Experimental Assessment of Ethanolic Extract of Moringa oleifera Leaves as an α-Amylase and α-Lipase Inhibitor Ogundipe, Adebanke Adetuyi, Babatunde Iheagwam, Franklyn Adefoyeke, Keleko Olugbuyiro, Joseph Ogunlana, Oluseyi Ogunlana, Olubanke Biochem Res Int Research Article METHODS: Phytochemical screening, antioxidant activity, α-amylase, and α-lipase inhibitory assessment were carried out on Moringa oleifera extract. RESULTS: The result of the phytochemical screening revealed the presence of total phenolic, flavonoid, tannin, and alkaloid contents of values 0.070 ± 0.005 mg gallic acid equivalent/g, 0.180 ± 0.020 mg rutin equivalent/g, 0.042 ± 0.001 mg tannic equivalent/g, and 12.17 ± 0.001%, respectively, while the total protein analysis was 0.475 ± 0.001 mg bovine serum albumin equivalent/g. Ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) values were 0.534 ± 0.001 mg gallic acid equivalent/g and 0.022 ± 0.00008 mg rutin equivalent/g, respectively. Diphenyl-2-picrylhydrazyl (DPPH), ABTS (2,2′-azino-bis (ethylbenzothiazoline-6-sulfonic acid)), and nitric oxide (NO) assays showed the extract to have a strong free radical scavenging activity. The 50% inhibitory concentration (IC(50)) values of the lipase and amylase activities of the extract are 1.0877 mg/mL and 0.1802 mg/mL, respectively. CONCLUSION: However, α-lipase and α-amylase inhibiting activity of M. oleifera could be related to the phytochemicals in the extract. This research validates the ethnobotanical use of M. oleifera leaves as an effective plant-based therapeutic agent for diabetes and obesity. Hindawi 2022-12-29 /pmc/articles/PMC9815922/ /pubmed/36620201 http://dx.doi.org/10.1155/2022/4613109 Text en Copyright © 2022 Adebanke Ogundipe et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ogundipe, Adebanke
Adetuyi, Babatunde
Iheagwam, Franklyn
Adefoyeke, Keleko
Olugbuyiro, Joseph
Ogunlana, Oluseyi
Ogunlana, Olubanke
In Vitro Experimental Assessment of Ethanolic Extract of Moringa oleifera Leaves as an α-Amylase and α-Lipase Inhibitor
title In Vitro Experimental Assessment of Ethanolic Extract of Moringa oleifera Leaves as an α-Amylase and α-Lipase Inhibitor
title_full In Vitro Experimental Assessment of Ethanolic Extract of Moringa oleifera Leaves as an α-Amylase and α-Lipase Inhibitor
title_fullStr In Vitro Experimental Assessment of Ethanolic Extract of Moringa oleifera Leaves as an α-Amylase and α-Lipase Inhibitor
title_full_unstemmed In Vitro Experimental Assessment of Ethanolic Extract of Moringa oleifera Leaves as an α-Amylase and α-Lipase Inhibitor
title_short In Vitro Experimental Assessment of Ethanolic Extract of Moringa oleifera Leaves as an α-Amylase and α-Lipase Inhibitor
title_sort in vitro experimental assessment of ethanolic extract of moringa oleifera leaves as an α-amylase and α-lipase inhibitor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9815922/
https://www.ncbi.nlm.nih.gov/pubmed/36620201
http://dx.doi.org/10.1155/2022/4613109
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