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Protein biosynthesis at the ER: finding the right accessories

More than 30% of eukaryotic proteins contain domains that must translocate across or integrate into the endoplasmic reticulum (ER) membrane. With few exceptions, protein translocation and transmembrane domain integration at the ER require the conserved Sec61 translocon. Decades of studies have estab...

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Autor principal: Shao, Sichen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816642/
https://www.ncbi.nlm.nih.gov/pubmed/36520029
http://dx.doi.org/10.1091/mbc.E21-09-0451
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author Shao, Sichen
author_facet Shao, Sichen
author_sort Shao, Sichen
collection PubMed
description More than 30% of eukaryotic proteins contain domains that must translocate across or integrate into the endoplasmic reticulum (ER) membrane. With few exceptions, protein translocation and transmembrane domain integration at the ER require the conserved Sec61 translocon. Decades of studies have established a clear mechanistic model for how the Sec61 translocon functions. The biosynthesis of distinct subsets of proteins at the ER also involves accessory factors that interact with the Sec61 translocon and translocating nascent proteins. However, assigning specific functions to many translocon accessory factors has been a persistent challenge in the field. This Perspective discusses recent insights into mechanisms that promote protein biosynthesis at the ER through accessory factors that directly regulate the Sec61 translocon or chaperone nascent proteins within the ER membrane. These translocon accessory factor functions, and more still to be discovered, are essential for producing a diverse and high-fidelity proteome at the ER.
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spelling pubmed-98166422023-03-02 Protein biosynthesis at the ER: finding the right accessories Shao, Sichen Mol Biol Cell Perspective More than 30% of eukaryotic proteins contain domains that must translocate across or integrate into the endoplasmic reticulum (ER) membrane. With few exceptions, protein translocation and transmembrane domain integration at the ER require the conserved Sec61 translocon. Decades of studies have established a clear mechanistic model for how the Sec61 translocon functions. The biosynthesis of distinct subsets of proteins at the ER also involves accessory factors that interact with the Sec61 translocon and translocating nascent proteins. However, assigning specific functions to many translocon accessory factors has been a persistent challenge in the field. This Perspective discusses recent insights into mechanisms that promote protein biosynthesis at the ER through accessory factors that directly regulate the Sec61 translocon or chaperone nascent proteins within the ER membrane. These translocon accessory factor functions, and more still to be discovered, are essential for producing a diverse and high-fidelity proteome at the ER. The American Society for Cell Biology 2022-12-15 /pmc/articles/PMC9816642/ /pubmed/36520029 http://dx.doi.org/10.1091/mbc.E21-09-0451 Text en © 2023 Shao. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License.
spellingShingle Perspective
Shao, Sichen
Protein biosynthesis at the ER: finding the right accessories
title Protein biosynthesis at the ER: finding the right accessories
title_full Protein biosynthesis at the ER: finding the right accessories
title_fullStr Protein biosynthesis at the ER: finding the right accessories
title_full_unstemmed Protein biosynthesis at the ER: finding the right accessories
title_short Protein biosynthesis at the ER: finding the right accessories
title_sort protein biosynthesis at the er: finding the right accessories
topic Perspective
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816642/
https://www.ncbi.nlm.nih.gov/pubmed/36520029
http://dx.doi.org/10.1091/mbc.E21-09-0451
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