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Protein biosynthesis at the ER: finding the right accessories
More than 30% of eukaryotic proteins contain domains that must translocate across or integrate into the endoplasmic reticulum (ER) membrane. With few exceptions, protein translocation and transmembrane domain integration at the ER require the conserved Sec61 translocon. Decades of studies have estab...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The American Society for Cell Biology
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816642/ https://www.ncbi.nlm.nih.gov/pubmed/36520029 http://dx.doi.org/10.1091/mbc.E21-09-0451 |
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author | Shao, Sichen |
author_facet | Shao, Sichen |
author_sort | Shao, Sichen |
collection | PubMed |
description | More than 30% of eukaryotic proteins contain domains that must translocate across or integrate into the endoplasmic reticulum (ER) membrane. With few exceptions, protein translocation and transmembrane domain integration at the ER require the conserved Sec61 translocon. Decades of studies have established a clear mechanistic model for how the Sec61 translocon functions. The biosynthesis of distinct subsets of proteins at the ER also involves accessory factors that interact with the Sec61 translocon and translocating nascent proteins. However, assigning specific functions to many translocon accessory factors has been a persistent challenge in the field. This Perspective discusses recent insights into mechanisms that promote protein biosynthesis at the ER through accessory factors that directly regulate the Sec61 translocon or chaperone nascent proteins within the ER membrane. These translocon accessory factor functions, and more still to be discovered, are essential for producing a diverse and high-fidelity proteome at the ER. |
format | Online Article Text |
id | pubmed-9816642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-98166422023-03-02 Protein biosynthesis at the ER: finding the right accessories Shao, Sichen Mol Biol Cell Perspective More than 30% of eukaryotic proteins contain domains that must translocate across or integrate into the endoplasmic reticulum (ER) membrane. With few exceptions, protein translocation and transmembrane domain integration at the ER require the conserved Sec61 translocon. Decades of studies have established a clear mechanistic model for how the Sec61 translocon functions. The biosynthesis of distinct subsets of proteins at the ER also involves accessory factors that interact with the Sec61 translocon and translocating nascent proteins. However, assigning specific functions to many translocon accessory factors has been a persistent challenge in the field. This Perspective discusses recent insights into mechanisms that promote protein biosynthesis at the ER through accessory factors that directly regulate the Sec61 translocon or chaperone nascent proteins within the ER membrane. These translocon accessory factor functions, and more still to be discovered, are essential for producing a diverse and high-fidelity proteome at the ER. The American Society for Cell Biology 2022-12-15 /pmc/articles/PMC9816642/ /pubmed/36520029 http://dx.doi.org/10.1091/mbc.E21-09-0451 Text en © 2023 Shao. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License. |
spellingShingle | Perspective Shao, Sichen Protein biosynthesis at the ER: finding the right accessories |
title | Protein biosynthesis at the ER: finding the right accessories |
title_full | Protein biosynthesis at the ER: finding the right accessories |
title_fullStr | Protein biosynthesis at the ER: finding the right accessories |
title_full_unstemmed | Protein biosynthesis at the ER: finding the right accessories |
title_short | Protein biosynthesis at the ER: finding the right accessories |
title_sort | protein biosynthesis at the er: finding the right accessories |
topic | Perspective |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9816642/ https://www.ncbi.nlm.nih.gov/pubmed/36520029 http://dx.doi.org/10.1091/mbc.E21-09-0451 |
work_keys_str_mv | AT shaosichen proteinbiosynthesisattheerfindingtherightaccessories |