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Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101

Pediococcus pentosaceus 1101 was identified by using 16S rRNA and MALDI-Biotyper. The strain was exposed to conditions that resemble the gastrointestinal tract (GT) to evaluate its probiotic properties. That included the growth kinetics, proteolytic and inhibitory activities within a pH range, survi...

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Autores principales: Escobar-Sánchez, Monserrat, Carrasco-Navarro, Ulises, Juárez-Castelán, Carmen, Lozano-Aguirre Beltrán, Luis, Pérez-Chabela, M. Lourdes, Ponce-Alquicira, Edith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818561/
https://www.ncbi.nlm.nih.gov/pubmed/36613263
http://dx.doi.org/10.3390/foods12010046
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author Escobar-Sánchez, Monserrat
Carrasco-Navarro, Ulises
Juárez-Castelán, Carmen
Lozano-Aguirre Beltrán, Luis
Pérez-Chabela, M. Lourdes
Ponce-Alquicira, Edith
author_facet Escobar-Sánchez, Monserrat
Carrasco-Navarro, Ulises
Juárez-Castelán, Carmen
Lozano-Aguirre Beltrán, Luis
Pérez-Chabela, M. Lourdes
Ponce-Alquicira, Edith
author_sort Escobar-Sánchez, Monserrat
collection PubMed
description Pediococcus pentosaceus 1101 was identified by using 16S rRNA and MALDI-Biotyper. The strain was exposed to conditions that resemble the gastrointestinal tract (GT) to evaluate its probiotic properties. That included the growth kinetics, proteolytic and inhibitory activities within a pH range, survival at low pH and in the presence of bile salts, antagonistic activity, cell-adhesion properties, and antibiotic resistance. The evaluation was followed by a genomic and proteomic analysis that involved the identification of proteins obtained under control and gastrointestinal conditions. The strain showed antagonistic activity against Gram-negative and Gram-positive bacteria, high resistance to acidity (87% logarithmic survival rate, pH 2) and bile salts (99% logarithmic survival rate, 0.5% w/v), and hydrophobic binding, as well as sensitivity to penicillin, amoxicillin, and chloramphenicol. On the other hand, P. pentosaceus 1101 has a genome size of 1.76 Mbp, with 1754 coding sequences, 55 rRNAs, and 33 tRNAs. The proteomic analysis showed that 120 proteins were involved in mechanisms in which the strain senses the effects of acid and bile salts. Moreover, the strain produces at least one lytic enzyme (N-acetylmuramoyl-L-alanine amidase; 32 kDa) that may be related to the antimicrobial activity. Therefore, proteins identified might be a key factor when it comes to the adaptation of P. pentosaceus 1101 into the GT and associated with its technological and probiotic properties.
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spelling pubmed-98185612023-01-07 Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101 Escobar-Sánchez, Monserrat Carrasco-Navarro, Ulises Juárez-Castelán, Carmen Lozano-Aguirre Beltrán, Luis Pérez-Chabela, M. Lourdes Ponce-Alquicira, Edith Foods Article Pediococcus pentosaceus 1101 was identified by using 16S rRNA and MALDI-Biotyper. The strain was exposed to conditions that resemble the gastrointestinal tract (GT) to evaluate its probiotic properties. That included the growth kinetics, proteolytic and inhibitory activities within a pH range, survival at low pH and in the presence of bile salts, antagonistic activity, cell-adhesion properties, and antibiotic resistance. The evaluation was followed by a genomic and proteomic analysis that involved the identification of proteins obtained under control and gastrointestinal conditions. The strain showed antagonistic activity against Gram-negative and Gram-positive bacteria, high resistance to acidity (87% logarithmic survival rate, pH 2) and bile salts (99% logarithmic survival rate, 0.5% w/v), and hydrophobic binding, as well as sensitivity to penicillin, amoxicillin, and chloramphenicol. On the other hand, P. pentosaceus 1101 has a genome size of 1.76 Mbp, with 1754 coding sequences, 55 rRNAs, and 33 tRNAs. The proteomic analysis showed that 120 proteins were involved in mechanisms in which the strain senses the effects of acid and bile salts. Moreover, the strain produces at least one lytic enzyme (N-acetylmuramoyl-L-alanine amidase; 32 kDa) that may be related to the antimicrobial activity. Therefore, proteins identified might be a key factor when it comes to the adaptation of P. pentosaceus 1101 into the GT and associated with its technological and probiotic properties. MDPI 2022-12-22 /pmc/articles/PMC9818561/ /pubmed/36613263 http://dx.doi.org/10.3390/foods12010046 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Escobar-Sánchez, Monserrat
Carrasco-Navarro, Ulises
Juárez-Castelán, Carmen
Lozano-Aguirre Beltrán, Luis
Pérez-Chabela, M. Lourdes
Ponce-Alquicira, Edith
Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101
title Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101
title_full Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101
title_fullStr Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101
title_full_unstemmed Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101
title_short Probiotic Properties and Proteomic Analysis of Pediococcus pentosaceus 1101
title_sort probiotic properties and proteomic analysis of pediococcus pentosaceus 1101
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9818561/
https://www.ncbi.nlm.nih.gov/pubmed/36613263
http://dx.doi.org/10.3390/foods12010046
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