Employing Engineered Enolase Promoter for Efficient Expression of Thermomyces lanuginosus Lipase in Yarrowia lipolytica via a Self-Excisable Vector

Yarrowia lipolytica is progressively being employed as a workhouse for recombinant protein expression. Here, we expanded the molecular toolbox by engineering the enolase promoter (pENO) and developed a new self-excisable vector, and based on this, a combined strategy was employed to enhance the expr...

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Detalles Bibliográficos
Autores principales: Jiao, Liangcheng, Li, Wenjuan, Li, Yunchong, Zhou, Qinghua, Zhu, Mengqin, Zhao, Guowei, Zhang, Houjin, Yan, Yunjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821249/
https://www.ncbi.nlm.nih.gov/pubmed/36614159
http://dx.doi.org/10.3390/ijms24010719

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9821249/
https://www.ncbi.nlm.nih.gov/pubmed/36614159
http://dx.doi.org/10.3390/ijms24010719

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