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Metabolic Profiles, Genetic Diversity, and Genome Size of Bulgarian Population of Alkanna tinctoria

Alkanna tinctoria (L.) Tausch Boraginaceae is a medicinal plant whose root is used for its antimicrobial and anti-inflammatory properties. A. tinctoria roots have been subject to numerous studies. However, the aerial parts have been explored less. The objective of the present study was to compare th...

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Autores principales: Nikolova, Milena, Aneva, Ina, Zhelev, Petar, Semerdjieva, Ivanka, Zheljazkov, Valtcho D., Vladimirov, Vladimir, Stoyanov, Stoyan, Berkov, Strahil, Yankova-Tsvetkova, Elina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823991/
https://www.ncbi.nlm.nih.gov/pubmed/36616241
http://dx.doi.org/10.3390/plants12010111
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author Nikolova, Milena
Aneva, Ina
Zhelev, Petar
Semerdjieva, Ivanka
Zheljazkov, Valtcho D.
Vladimirov, Vladimir
Stoyanov, Stoyan
Berkov, Strahil
Yankova-Tsvetkova, Elina
author_facet Nikolova, Milena
Aneva, Ina
Zhelev, Petar
Semerdjieva, Ivanka
Zheljazkov, Valtcho D.
Vladimirov, Vladimir
Stoyanov, Stoyan
Berkov, Strahil
Yankova-Tsvetkova, Elina
author_sort Nikolova, Milena
collection PubMed
description Alkanna tinctoria (L.) Tausch Boraginaceae is a medicinal plant whose root is used for its antimicrobial and anti-inflammatory properties. A. tinctoria roots have been subject to numerous studies. However, the aerial parts have been explored less. The objective of the present study was to compare the chemical profile of aerial parts and roots as well as the total alkannin content in roots of 11 populations of the species from different floristic regions of Bulgaria. Methanolic extracts from 22 samples were analyzed by GC/MS. Phenolic, fatty, and organic acids, sterols, polyols, fatty alcohols, and sugars were identified. Ononitol (4-O-methyl-myo-inositol) was found as the main compound in the aerial parts. The total alkannin content in the roots was evaluated by the spectrophotometric method and compared with that of the commercial product. Populations with high alkannin content and rich in other bioactive compounds were identified. A relatively low genetic diversity in the studied populations was observed. The present study is the first comprehensive study on metabolite profiles and genetic diversity of the Bulgarian populations of A. tinctoria. The occurrence of ononitol in the aerial parts of the species is reported for the first time, as well as the phenolic acid profiles of the species in both aerial parts and roots. The results showed that aerial parts of the plant are also promising for use as a source of valuable biologically active substances.
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spelling pubmed-98239912023-01-08 Metabolic Profiles, Genetic Diversity, and Genome Size of Bulgarian Population of Alkanna tinctoria Nikolova, Milena Aneva, Ina Zhelev, Petar Semerdjieva, Ivanka Zheljazkov, Valtcho D. Vladimirov, Vladimir Stoyanov, Stoyan Berkov, Strahil Yankova-Tsvetkova, Elina Plants (Basel) Article Alkanna tinctoria (L.) Tausch Boraginaceae is a medicinal plant whose root is used for its antimicrobial and anti-inflammatory properties. A. tinctoria roots have been subject to numerous studies. However, the aerial parts have been explored less. The objective of the present study was to compare the chemical profile of aerial parts and roots as well as the total alkannin content in roots of 11 populations of the species from different floristic regions of Bulgaria. Methanolic extracts from 22 samples were analyzed by GC/MS. Phenolic, fatty, and organic acids, sterols, polyols, fatty alcohols, and sugars were identified. Ononitol (4-O-methyl-myo-inositol) was found as the main compound in the aerial parts. The total alkannin content in the roots was evaluated by the spectrophotometric method and compared with that of the commercial product. Populations with high alkannin content and rich in other bioactive compounds were identified. A relatively low genetic diversity in the studied populations was observed. The present study is the first comprehensive study on metabolite profiles and genetic diversity of the Bulgarian populations of A. tinctoria. The occurrence of ononitol in the aerial parts of the species is reported for the first time, as well as the phenolic acid profiles of the species in both aerial parts and roots. The results showed that aerial parts of the plant are also promising for use as a source of valuable biologically active substances. MDPI 2022-12-26 /pmc/articles/PMC9823991/ /pubmed/36616241 http://dx.doi.org/10.3390/plants12010111 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nikolova, Milena
Aneva, Ina
Zhelev, Petar
Semerdjieva, Ivanka
Zheljazkov, Valtcho D.
Vladimirov, Vladimir
Stoyanov, Stoyan
Berkov, Strahil
Yankova-Tsvetkova, Elina
Metabolic Profiles, Genetic Diversity, and Genome Size of Bulgarian Population of Alkanna tinctoria
title Metabolic Profiles, Genetic Diversity, and Genome Size of Bulgarian Population of Alkanna tinctoria
title_full Metabolic Profiles, Genetic Diversity, and Genome Size of Bulgarian Population of Alkanna tinctoria
title_fullStr Metabolic Profiles, Genetic Diversity, and Genome Size of Bulgarian Population of Alkanna tinctoria
title_full_unstemmed Metabolic Profiles, Genetic Diversity, and Genome Size of Bulgarian Population of Alkanna tinctoria
title_short Metabolic Profiles, Genetic Diversity, and Genome Size of Bulgarian Population of Alkanna tinctoria
title_sort metabolic profiles, genetic diversity, and genome size of bulgarian population of alkanna tinctoria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9823991/
https://www.ncbi.nlm.nih.gov/pubmed/36616241
http://dx.doi.org/10.3390/plants12010111
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