Preparation of Functionalized Magnetic Polystyrene Microspheres and Their Application in Food Safety Detection

Based on the specific binding of sulfonic acid groups to melamine, β-agonists and other compounds, Fe(3)O(4) nano-magnetic beads were coated with polystyrene using an improved micro-suspension emulsion polymerization method, thus forming core–shell magnetic polystyrene microspheres (Fe(3)O(4)@PS) with Fe(3)O(4) as the core and polystyrene as the shell. These functionalized microspheres, which can be used as magnetic solid-phase extraction (MSPE) adsorbent, were prepared after further sulfonation. These microspheres were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), particle size analysis and saturation magnetization measurement. The results showed that these sulfonated magnetic polystyrene microspheres had favorable sphericity. The particle size of these microspheres ranged from 1 μm to 10 μm. Additionally, these microspheres had good dispersion and magnetic responses in both inorganic and organic solvents. Moreover, these functionalized magnetic polystyrene microspheres were tested and evaluated by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The results indicated that these sulfonated magnetic polystyrene microspheres (Fe(3)O(4)@SPS) could effectively adsorb such illegal additives as β-agonists and melamine in the food matrix.