CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids

Antibiotics have been widely used for plasmid-mediated cell engineering. However, continued use of antibiotics increases the metabolic burden, horizontal gene transfer risks, and biomanufacturing costs. There are limited approaches to maintaining multiple plasmids without antibiotics. Herein, we dev...

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Detalles Bibliográficos
Autores principales: Kim, Seong Keun, Kim, Haseong, Woo, Seung Gyun, Kim, Tae Hyun, Rha, Eugene, Kwon, Kil Koang, Lee, Hyewon, Lee, Seung-Goo, Lee, Dae-Hee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825151/
https://www.ncbi.nlm.nih.gov/pubmed/36511859
http://dx.doi.org/10.1093/nar/gkac1104

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825151/
https://www.ncbi.nlm.nih.gov/pubmed/36511859
http://dx.doi.org/10.1093/nar/gkac1104

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