Cargando…
CRISPRi-based programmable logic inverter cascade for antibiotic-free selection and maintenance of multiple plasmids
Antibiotics have been widely used for plasmid-mediated cell engineering. However, continued use of antibiotics increases the metabolic burden, horizontal gene transfer risks, and biomanufacturing costs. There are limited approaches to maintaining multiple plasmids without antibiotics. Herein, we dev...
| Autores principales: | , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9825151/ https://www.ncbi.nlm.nih.gov/pubmed/36511859 http://dx.doi.org/10.1093/nar/gkac1104 |