Cargando…

Two Tags in One Probe: Combining Fluorescence‐ and Biotin‐based Detection of the Trypanosomal Cysteine Protease Rhodesain

Rhodesain is the major cysteine protease of the protozoan parasite Trypanosoma brucei and a therapeutic target for sleeping sickness, a fatal neglected tropical disease. We designed, synthesized and characterized a bimodal activity‐based probe that binds to and inactivates rhodesain. This probe exhi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lemke, Carina, Jílková, Adéla, Ferber, Dominic, Braune, Annett, On, Anja, Johe, Patrick, Zíková, Alena, Schirmeister, Tanja, Mareš, Michael, Horn, Martin, Gütschow, Michael
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2022
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826439/ https://www.ncbi.nlm.nih.gov/pubmed/35852812 http://dx.doi.org/10.1002/chem.202201636

Ejemplares similares

Structure, interdomain dynamics, and pH-dependent autoactivation of pro-rhodesain, the main lysosomal cysteine protease from African trypanosomes
por: Johé, Patrick, et al.
Publicado: (2021)

New Cysteine Protease Inhibitors: Electrophilic (Het)arenes and Unexpected Prodrug Identification for the Trypanosoma Protease Rhodesain
por: Klein, Philipp, et al.
Publicado: (2020)

Development of Urea-Bond-Containing Michael Acceptors as Antitrypanosomal Agents Targeting Rhodesain
por: Previti, Santo, et al.
Publicado: (2022)

On the Reversible and Irreversible Inhibition of Rhodesain by Curcumin
por: Steverding, Dietmar
Publicado: (2019)

Crystal Structures of TbCatB and Rhodesain, Potential Chemotherapeutic Targets and Major Cysteine Proteases of Trypanosoma brucei
por: Kerr, Iain D., et al.
Publicado: (2010)

Dipeptide Nitrile CD34 with Curcumin: A New Improved Combination Strategy to Synergistically Inhibit Rhodesain of Trypanosoma brucei rhodesiense
por: Di Chio, Carla, et al.
Publicado: (2023)

Trypanosome Mitochondrial Translation and Tetracycline: No Sweat about Tet
por: Hashimi, Hassan, et al.
Publicado: (2016)

Redesigned and reversed: architectural and functional oddities of the trypanosomal ATP synthase
por: Gahura, Ondřej, et al.
Publicado: (2021)

Drug Combination Studies of the Dipeptide Nitrile CD24 with Curcumin: A New Strategy to Synergistically Inhibit Rhodesain of Trypanosoma brucei rhodesiense
por: Di Chio, Carla, et al.
Publicado: (2022)

ATPaseTb2, a Unique Membrane-bound FoF1-ATPase Component, Is Essential in Bloodstream and Dyskinetoplastic Trypanosomes
por: Šubrtová, Karolína, et al.
Publicado: (2015)

Suramin exposure alters cellular metabolism and mitochondrial energy production in African trypanosomes
por: Zoltner, Martin, et al.
Publicado: (2020)

Synthesis of Biotin-Tagged Chitosan Oligosaccharides and Assessment of Their Immunomodulatory Activity
por: Tsvetkov, Yury E., et al.
Publicado: (2020)

The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation
por: van Werven, Folkert J., et al.
Publicado: (2006)

GATS tag system is compatible with biotin labelling methods for protein analysis
por: Yamada, Kohdai, et al.
Publicado: (2023)

Telomeres, tethers and trypanosomes
por: Field, Mark C., et al.
Publicado: (2012)

Naphthoquinones as Covalent Reversible Inhibitors of Cysteine Proteases—Studies on Inhibition Mechanism and Kinetics
por: Klein, Philipp, et al.
Publicado: (2020)

Development of Fluorescent and Biotin Probes Targeting NLRP3
por: Keuler, Tim, et al.
Publicado: (2021)

Azanitrile Inhibitors of the SmCB1 Protease Target Are Lethal to Schistosoma mansoni: Structural and Mechanistic Insights into Chemotype Reactivity
por: Jílková, Adéla, et al.
Publicado: (2020)

Optimal use of tandem biotin and V5 tags in ChIP assays
por: Kolodziej, Katarzyna E, et al.
Publicado: (2009)

GSH Activated Biotin-tagged Near-Infrared Probe for Efficient Cancer Imaging
por: Guo, Ruiying, et al.
Publicado: (2019)

Antigenic variation in African trypanosomes
por: Horn, David
Publicado: (2014)

Anti-trypanosomal activity of non-peptidic nitrile-based cysteine protease inhibitors
por: Burtoloso, Antonio C. B., et al.
Publicado: (2017)

3′-Biotin-tagged microRNA-27 does not associate with Argonaute proteins in cells
por: Guo, Yang Eric, et al.
Publicado: (2014)

Biotin proximity tagging favours unfolded proteins and enables the study of intrinsically disordered regions
por: Minde, David-Paul, et al.
Publicado: (2020)

PNT1 Is a C11 Cysteine Peptidase Essential for Replication of the Trypanosome Kinetoplast
por: Grewal, Jaspreet S., et al.
Publicado: (2016)

TrypTag.org: A Trypanosome Genome-wide Protein Localisation Resource
por: Dean, Samuel, et al.
Publicado: (2017)

New Tetromycin Derivatives with Anti-Trypanosomal and Protease Inhibitory Activities
por: Pimentel-Elardo, Sheila M., et al.
Publicado: (2011)

Force Dependent Biotinylation of Myosin IIA by α-Catenin Tagged with a Promiscuous Biotin Ligase
por: Ueda, Shuji, et al.
Publicado: (2015)

Biotin tagging of MeCP2 in mice reveals contextual insights into the Rett syndrome transcriptome
por: Johnson, B.S., et al.
Publicado: (2017)

Indolizine-Based Scaffolds as Efficient and Versatile Tools: Application to the Synthesis of Biotin-Tagged Antiangiogenic Drugs
por: Arvin-Berod, Marie, et al.
Publicado: (2017)

Procyclic trypanosomes recycle glucose catabolites and TCA cycle intermediates to stimulate growth in the presence of physiological amounts of proline
por: Villafraz, Oriana, et al.
Publicado: (2021)

A unique mRNA decapping complex in trypanosomes
por: Kramer, Susanne, et al.
Publicado: (2023)

Mapping of O-GlcNAc Sites of 20 S Proteasome Subunits and Hsp90 by a Novel Biotin-Cystamine Tag
por: Overath, Thorsten, et al.
Publicado: (2012)

Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli
por: Delli-Bovi, Teegan A, et al.
Publicado: (2010)

Biotin-tagged proteins: Reagents for efficient ELISA-based serodiagnosis and phage display-based affinity selection
por: Verma, Vaishali, et al.
Publicado: (2018)

Genetic dissection of drug resistance in trypanosomes
por: ALSFORD, SAM, et al.
Publicado: (2013)

Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism
por: Belda, Eugeni, et al.
Publicado: (2022)

A biotin-streptavidin-biotin bridge dramatically enhances cell fusion
por: LI, JINHUA, et al.
Publicado: (2014)

Biotin Auxotrophy and Biotin Enhanced Germ Tube Formation in Candida albicans
por: Ahmad Hussin, Nur, et al.
Publicado: (2016)

Development and Evaluation of an Anti-Biotin Interference Method in Biotin-Streptavidin Immunoassays
por: Liu, Dong, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_nt45tctv6rjo0ld5cpvg07i9de