Proximity biotinylation to define the local environment of the protein kinase A catalytic subunit in adrenal cells

Mutant protein kinase A catalytic subunit (PKAc) drives adrenal Cushing’s syndrome, though its signaling interactions remain unclear. This protocol details steps to use live-cell proximity labeling to identify subcellular compartments and proteins closely associated with variants of PKAc in human ad...

Descripción completa

Detalles Bibliográficos
Autores principales: Omar, Mitchell H., Lauer, Sophia M., Lau, Ho-Tak, Golkowski, Martin, Ong, Shao-En, Scott, John D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826815/
https://www.ncbi.nlm.nih.gov/pubmed/36607814
http://dx.doi.org/10.1016/j.xpro.2022.101992

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826815/
https://www.ncbi.nlm.nih.gov/pubmed/36607814
http://dx.doi.org/10.1016/j.xpro.2022.101992

Ejemplares similares