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Proximity biotinylation to define the local environment of the protein kinase A catalytic subunit in adrenal cells

Mutant protein kinase A catalytic subunit (PKAc) drives adrenal Cushing’s syndrome, though its signaling interactions remain unclear. This protocol details steps to use live-cell proximity labeling to identify subcellular compartments and proteins closely associated with variants of PKAc in human ad...

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Detalles Bibliográficos
Autores principales:	Omar, Mitchell H., Lauer, Sophia M., Lau, Ho-Tak, Golkowski, Martin, Ong, Shao-En, Scott, John D.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826815/ https://www.ncbi.nlm.nih.gov/pubmed/36607814 http://dx.doi.org/10.1016/j.xpro.2022.101992

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