Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures

Several pre-clinical models reveal that chronic chimeric antigen receptor (CAR) stimulation drives a dysfunctional state that mimics in vivo failure. In this protocol, we describe steps to induce T cell dysfunction by persistent and long-term stimulation of CAR-engineered T cells using antigen-expre...

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Detalles Bibliográficos
Autores principales: Selli, Mehmet Emrah, Landmann, Jack H., Arveseth, Corvin, Singh, Nathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826863/
https://www.ncbi.nlm.nih.gov/pubmed/36607811
http://dx.doi.org/10.1016/j.xpro.2022.101954
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author Selli, Mehmet Emrah
Landmann, Jack H.
Arveseth, Corvin
Singh, Nathan
author_facet Selli, Mehmet Emrah
Landmann, Jack H.
Arveseth, Corvin
Singh, Nathan
author_sort Selli, Mehmet Emrah
collection PubMed
description Several pre-clinical models reveal that chronic chimeric antigen receptor (CAR) stimulation drives a dysfunctional state that mimics in vivo failure. In this protocol, we describe steps to induce T cell dysfunction by persistent and long-term stimulation of CAR-engineered T cells using antigen-expressing cancer cells in suspension cultures. We first described a validated method for manufacturing of CAR T cells, followed by a detailed method for chronic stimulation of CAR T cells and a strategy to evaluate these cells during the process of chronic stimulation. For complete details on the use and execution of this protocol, please refer to Singh et al. (2020).(1)
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spelling pubmed-98268632023-01-10 Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures Selli, Mehmet Emrah Landmann, Jack H. Arveseth, Corvin Singh, Nathan STAR Protoc Protocol Several pre-clinical models reveal that chronic chimeric antigen receptor (CAR) stimulation drives a dysfunctional state that mimics in vivo failure. In this protocol, we describe steps to induce T cell dysfunction by persistent and long-term stimulation of CAR-engineered T cells using antigen-expressing cancer cells in suspension cultures. We first described a validated method for manufacturing of CAR T cells, followed by a detailed method for chronic stimulation of CAR T cells and a strategy to evaluate these cells during the process of chronic stimulation. For complete details on the use and execution of this protocol, please refer to Singh et al. (2020).(1) Elsevier 2023-01-04 /pmc/articles/PMC9826863/ /pubmed/36607811 http://dx.doi.org/10.1016/j.xpro.2022.101954 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Selli, Mehmet Emrah
Landmann, Jack H.
Arveseth, Corvin
Singh, Nathan
Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures
title Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures
title_full Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures
title_fullStr Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures
title_full_unstemmed Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures
title_short Inducing T cell dysfunction by chronic stimulation of CAR-engineered T cells targeting cancer cells in suspension cultures
title_sort inducing t cell dysfunction by chronic stimulation of car-engineered t cells targeting cancer cells in suspension cultures
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826863/
https://www.ncbi.nlm.nih.gov/pubmed/36607811
http://dx.doi.org/10.1016/j.xpro.2022.101954
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