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An optogenetic tool to inhibit RhoA in Drosophila embryos

We describe a protocol for optogenetic inhibition of the small GTPase Rho1 (RhoA) in Drosophila embryos, which allows rapid and spatially confined inactivation of Rho1 and Rho1-mediated actomyosin contractility. We provide step-by-step instruction for optogenetic manipulations of Drosophila embryos...

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Detalles Bibliográficos
Autores principales: Guo, Hanqing, Swan, Michael, He, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826882/
https://www.ncbi.nlm.nih.gov/pubmed/36598852
http://dx.doi.org/10.1016/j.xpro.2022.101972