An optogenetic tool to inhibit RhoA in Drosophila embryos

We describe a protocol for optogenetic inhibition of the small GTPase Rho1 (RhoA) in Drosophila embryos, which allows rapid and spatially confined inactivation of Rho1 and Rho1-mediated actomyosin contractility. We provide step-by-step instruction for optogenetic manipulations of Drosophila embryos...

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Detalles Bibliográficos
Autores principales: Guo, Hanqing, Swan, Michael, He, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826882/
https://www.ncbi.nlm.nih.gov/pubmed/36598852
http://dx.doi.org/10.1016/j.xpro.2022.101972
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author Guo, Hanqing
Swan, Michael
He, Bing
author_facet Guo, Hanqing
Swan, Michael
He, Bing
author_sort Guo, Hanqing
collection PubMed
description We describe a protocol for optogenetic inhibition of the small GTPase Rho1 (RhoA) in Drosophila embryos, which allows rapid and spatially confined inactivation of Rho1 and Rho1-mediated actomyosin contractility. We provide step-by-step instruction for optogenetic manipulations of Drosophila embryos using confocal and multiphoton imaging systems. This tool is useful for determining the site- and stage-specific function of Rho1 in Drosophila embryos and for studying the immediate tissue response to acute elimination of cellular contractility. For complete details on the use and execution of this protocol, please refer to Guo et al. (2022).(1)
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spelling pubmed-98268822023-01-10 An optogenetic tool to inhibit RhoA in Drosophila embryos Guo, Hanqing Swan, Michael He, Bing STAR Protoc Protocol We describe a protocol for optogenetic inhibition of the small GTPase Rho1 (RhoA) in Drosophila embryos, which allows rapid and spatially confined inactivation of Rho1 and Rho1-mediated actomyosin contractility. We provide step-by-step instruction for optogenetic manipulations of Drosophila embryos using confocal and multiphoton imaging systems. This tool is useful for determining the site- and stage-specific function of Rho1 in Drosophila embryos and for studying the immediate tissue response to acute elimination of cellular contractility. For complete details on the use and execution of this protocol, please refer to Guo et al. (2022).(1) Elsevier 2023-01-03 /pmc/articles/PMC9826882/ /pubmed/36598852 http://dx.doi.org/10.1016/j.xpro.2022.101972 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Guo, Hanqing
Swan, Michael
He, Bing
An optogenetic tool to inhibit RhoA in Drosophila embryos
title An optogenetic tool to inhibit RhoA in Drosophila embryos
title_full An optogenetic tool to inhibit RhoA in Drosophila embryos
title_fullStr An optogenetic tool to inhibit RhoA in Drosophila embryos
title_full_unstemmed An optogenetic tool to inhibit RhoA in Drosophila embryos
title_short An optogenetic tool to inhibit RhoA in Drosophila embryos
title_sort optogenetic tool to inhibit rhoa in drosophila embryos
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826882/
https://www.ncbi.nlm.nih.gov/pubmed/36598852
http://dx.doi.org/10.1016/j.xpro.2022.101972
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