An optimized iDISCO+ protocol for tissue clearing and 3D analysis of oxytocin and vasopressin cell network in the developing mouse brain

Here, we present an optimized iDISCO+ protocol combining tissue clearing and light sheet microscopy to map the postnatal development of oxytocin and vasopressin neurons in mouse hypothalamus. We describe tissue preparation, immunostaining, clearing, and imaging. We then detail how to process the 3D...

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Detalles Bibliográficos
Autores principales: Habart, Marie, Lio, Guillaume, Soumier, Amélie, Demily, Caroline, Sirigu, Angela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826885/
https://www.ncbi.nlm.nih.gov/pubmed/36598854
http://dx.doi.org/10.1016/j.xpro.2022.101968

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826885/
https://www.ncbi.nlm.nih.gov/pubmed/36598854
http://dx.doi.org/10.1016/j.xpro.2022.101968

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