Measuring SERCA-mediated calcium uptake in mouse muscle homogenates

This protocol employs the indo-1 Ca(2+) fluorophore to quantify Ca(2+) uptake by the sarco(endo)plasmic reticulum Ca(2+) ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca(2+) mobilization within the muscle homogenate. This protocol can be easily adapted for...

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Detalles Bibliográficos
Autores principales: Geromella, Mia S., Braun, Jessica L., Fajardo, Val A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826970/
https://www.ncbi.nlm.nih.gov/pubmed/36602905
http://dx.doi.org/10.1016/j.xpro.2022.101987

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826970/
https://www.ncbi.nlm.nih.gov/pubmed/36602905
http://dx.doi.org/10.1016/j.xpro.2022.101987

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