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Measuring SERCA-mediated calcium uptake in mouse muscle homogenates
This protocol employs the indo-1 Ca(2+) fluorophore to quantify Ca(2+) uptake by the sarco(endo)plasmic reticulum Ca(2+) ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca(2+) mobilization within the muscle homogenate. This protocol can be easily adapted for...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826970/ https://www.ncbi.nlm.nih.gov/pubmed/36602905 http://dx.doi.org/10.1016/j.xpro.2022.101987 |