Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9

While readily achieved in cell lines, the application of CRISPR-Cas9 gene editing in human-derived organoids suffers from limited efficacy and complex protocols. Here, we describe a multi-guide RNA CRISPR-Cas9 gene-editing protocol which efficiently achieves complete gene knockout in adult human col...

Descripción completa

Detalles Bibliográficos
Autores principales: Chan, Dedrick Kok Hong, Collins, Scott David, Buczacki, Simon James Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826973/
https://www.ncbi.nlm.nih.gov/pubmed/36598849
http://dx.doi.org/10.1016/j.xpro.2022.101978

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9826973/
https://www.ncbi.nlm.nih.gov/pubmed/36598849
http://dx.doi.org/10.1016/j.xpro.2022.101978

Ejemplares similares