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An Argonaute from Thermus parvatiensis exhibits endonuclease activity mediated by 5′ chemically modified DNA guides : Argonaute using 5′ chemically modified DNA guides
Prokaryotic Argonaute (pAgo) nucleases with precise DNA cleavage activity show great potential for gene manipulation. Extensive biochemical studies have revealed that recognition of guides with different 5′ groups by Ago is important for biocatalysis. Here, we identified an Ago from the thermophilic...
Autores principales: | Sun, Yingying, Guo, Xiang, Lu, Hui, Chen, Liuqing, Huang, Fei, Liu, Qian, Feng, Yan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828299/ https://www.ncbi.nlm.nih.gov/pubmed/35643958 http://dx.doi.org/10.3724/abbs.2022047 |
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