Lambda‐PCR for precise DNA assembly and modification

Lambda‐polymerase chain reaction (λ‐PCR) is a novel and open‐source method for DNA assembly and cloning projects. λ‐PCR uses overlap extension to ultimately assemble linear and circular DNA fragments, but it allows the single‐stranded DNA (ssDNA) primers of the PCR extension to first exist as double...

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Detalles Bibliográficos
Autores principales: Tanniche, Imen, Fisher, Amanda K., Gillam, Frank, Collakova, Eva, Zhang, Chenming, Bevan, David R., Senger, Ryan S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
ARTICLES
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828557/
https://www.ncbi.nlm.nih.gov/pubmed/36148504
http://dx.doi.org/10.1002/bit.28240

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9828557/
https://www.ncbi.nlm.nih.gov/pubmed/36148504
http://dx.doi.org/10.1002/bit.28240

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