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Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01

CRISPR/Cas9-mediated homology-directed recombination is an efficient method to express target genes. Based on the above method, providing ideal neutral integration sites can ensure the reliable, stable, and high expression of target genes. In this study, we obtained a fluorescent transformant with n...

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Detalles Bibliográficos
Autores principales: Tong, Sheng, An, Kexin, Chen, Wuxi, Chai, Mengdan, Sun, Yuanxia, Wang, Qinhong, Li, Demao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9830034/
https://www.ncbi.nlm.nih.gov/pubmed/36687472
http://dx.doi.org/10.1016/j.synbio.2022.12.006