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Identification of neutral genome integration sites with high expression and high integration efficiency in Fusarium venenatum TB01
CRISPR/Cas9-mediated homology-directed recombination is an efficient method to express target genes. Based on the above method, providing ideal neutral integration sites can ensure the reliable, stable, and high expression of target genes. In this study, we obtained a fluorescent transformant with n...
Autores principales: | Tong, Sheng, An, Kexin, Chen, Wuxi, Chai, Mengdan, Sun, Yuanxia, Wang, Qinhong, Li, Demao |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9830034/ https://www.ncbi.nlm.nih.gov/pubmed/36687472 http://dx.doi.org/10.1016/j.synbio.2022.12.006 |
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